Product Name:
Rat Ovalbumin Specific IgG (OVA-sIgG) ELISA Kit
Cat.#:
JL54322
Regulatory Status:
Alternative:
ovalbumin specific IgG
Detection Method:
ELISA
Assay Type:
Indirect
Detection Range:
Qualitative analysis
Sensitivity:
Specificity:
This kit recognizes Rat OVA-sIgG in samples. No significant cross-reactivity or interference between Rat OVA-sIgG and analogues was observed.
Sample Type:
Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids
Assay Procedure:
Research Area:
Immunology
Product Datasheets
| OVA-sIgG
Ovalbumin (abbreviated OVA) is the main protein found in egg white, making up approximately 55% of the total protein.Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine protease inhibitor.The ovalbumin protein of chickens consists of 385 amino acids, its relative molecular mass is 42.7 kDa,and it adopts a serpin-like structure.Ovalbumin also has several modifications, including N-terminal acetylation (G1), phosphorylation (S68, S344), and glycosylation (N292).[5] It has three isoforms, A1, A2, and A3, which vary based on the number of bound phosphate residues.| Typical data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.
| Repeatability
Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%| Recovery
The recovery of Rat OVA-sIgG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.| Linearity
Samples were spiked with high concentrations of Rat OVA-sIgG and diluted with Universal Diluent to produce samples with values within the range of the assay.
Size
Quantity
Price ($)
48T
1
396 / 396
96T
1
516 / 516


